Starch Blocking Stability of the Phaseolus vulgaris Alpha-Amylase Inhibitor (α-AI1)
نویسندگان
چکیده
منابع مشابه
Activation of bean (Phaseolus vulgaris) alpha-amylase inhibitor requires proteolytic processing of the proprotein.
Seeds of the common bean (Phaseolus vulgaris) contain a plant defense protein that inhibits the alpha-amylases of mammals and insects. This alpha-amylase inhibitor (alpha AI) is synthesized as a proprotein on the endoplasmic reticulum and is proteolytically processed after arrival in the protein storage vacuoles to polypeptides of relative molecular weight (M(r)) 15,000 to 18,000. We report two...
متن کاملPurification and properties of phaseolamin, an inhibitor of alpha-amylase, from the kidney bean, Phaseolus vulgaris.
Kidney beans, Phaseolus vulgaris, contain a proteinaceous inhibitor of alpha-amylase, which we have named phaseolamin. The inhibitor has been purified to homogeneity by conventional protein fractionation methods involving heat treatment, dialysis, and chromatography on DEAE-cellulose, Sephadex G-100, and CM-cellulose. Phaseolamin is specific for animal alpha-amylases, having no activity towards...
متن کاملActivity changes in cranberry bean (Phaseolus vulgaris) alpha-amylase inhibitor by chemical modification and enzymatic digestion.
An alpha-amylase inhibitor was prepared from cranberry bean (Phaseolus vulgaris). The alpha-amylase inhibitor was composed of three different subunits not linked by disulfide bridges and only one of them contained carbohydrate. Although the inhibitor was stable at pH 3 to 7, it was heat labile at pH 3 and 5. Chemical modification of the amino groups and the guanido groups in cranberry bean alph...
متن کاملReview Article The nutraceutical role of the Phaseolus vulgaris a-amylase inhibitor
The present review assesses the potential of the Phaseolus vulgaris a-amylase inhibitor isoform 1 (a-AI1) starch blockers as a widely used remedy against obesity and diabetes. Consumption of the a-amylase inhibitor causes marginal intraluminal a-amylase activity facilitated by the inhibitor’s appropriate structural, physico-chemical and functional properties. As a result there is decreased post...
متن کاملHeterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae
BACKGROUND Phaseolamin or α-amylase inhibitor 1 (αAI) is a glycoprotein from common beans (Phaseolus vulgaris L.) that inhibits some insect and mammalian α-amylases. Several clinical studies support the beneficial use of bean αAI for control of diabetes and obesity. Commercial extracts of P. vulgaris are available but their efficacy is still under question, mainly because some of these extracts...
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ژورنال
عنوان ژورنال: American Journal of Food Technology
سال: 2008
ISSN: 1557-4571
DOI: 10.3923/ajft.2009.9.19